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. Author manuscript; available in PMC: 2022 Jan 25.
Published in final edited form as: J Vis Exp. 2020 Jun 7;(160):10.3791/61272. doi: 10.3791/61272

Figure 1: Stereotactic and anesthesia systems.

Figure 1:

(A) Anesthesia induction chamber setup within a biological safety cabinet. (B) Stereotactic anesthetic delivery setup highlighting anesthesia tubing from the anesthetic machine to the nose cone on the stereotactic apparatus (see inset in (C)). Green arrows indicate delivered anesthetic gas tubing, and blue arrows indicate scavenged gas tubing. (C) Stereotactic stand with drill attachment. Inset shows anesthetic tubing (green and blue arrows), mouth bar, and ear bars. (D) Stereotactic stand with automated syringe pump and mouse elevating box. The box is an inverted pipette tip box containing hand warmers used to elevate the mouse to the appropriate height and maintain body temperature. Inset shows the orientation of the Hamilton syringe in the automated injection apparatus.