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Knockout fragment is stable despite fitness decrease. (A) Characterization of genetic reversion at the gut1 locus. Cells were grown overnight in YPD medium and stamped onto minimal glycerol agar medium. (B) Growth of engineered strains through serial passaging in 3 mL of plate culture. Error bars represent standard deviations across three biological replicates, passaged individually. (C) Growth of engineered strains through serial passaging in 200 mL flask culture.
