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. 2022 Jan 4;11(1):241–253. doi: 10.1021/acssynbio.1c00393

Figure 1.

Figure 1

Commonly used genetic cassettes encoding the TEM-1 β-lactamase. (A) Tn3 fragment from the R1 plasmid of S. paratyphi B (herein called Tn3.1) is 1216 nucleotides long. It contains the bla coding sequence as well as 5′ and 3′UTRs. The 5′UTR contains a P3 promoter and Shine–Dalgarno sequence. Numbers correspond to the AUG start codon of bla. The full sequence of Tn3.1 is shown in Table 1. (B) Versions of the Tn3.1 fragment that are truncated in the 5′ and 3′ UTR are commonly used (herein called Tn3.2–Tn3.17). These versions may also contain nucleotide substitutions (summarized in Table 1). (C) The pSEVA collection contains a codon-varied genetic module encoding the TEM-1 β-lactamase and is flanked by transcriptional terminators and restriction enzyme recognition sites (D). Estimated frequency in the literature of the Tn3-based fragments. Expression plasmids containing the various fragments were searched on Google Scholar, and the number of citations was recorded.