Table 1. Tn3 Fragments Used in Commercial Expression Plasmidsa.
| fragment | lengthQ | sequenceR | citationsS | expression plasmids using the fragment |
|---|---|---|---|---|
| Tn3.1 | 1–1216 | A | 246 266 | pBR332, pET1*, pET2*, pET3*, pET4*, pET5*, pET6*, pET7*, pET8*, pET11a-d, pET14b, pET15b, pET16b, pET17b, pET19b, pET-DEST42, pET100/D-TOPO, pET100/D-LacZ, pET101/D-LacZ, pET101/D-TOPO, pET102/D-LacZ, pET102/D-TOPO, pET104-DEST, pET104/GW/LacZ, pET104.1-DEST, pET104.1/D/GW-LacZ, pET151/D-TOPO, pET151/D/LacZ, pET160-DEST, pET160/GW/D-TOPO, pET161/GW-CAT, pET300/NT-GW/Ras Kinase, pET300/NT-DEST, pET301/CT-DEST, pET302/NT-his, pGEX-1 lambda T, pGEX-2T, pGEX-2TK, pGEX-3X, pGEX-4T-1, pGEX-4T-2, pGEX-4T-3, pGEX-5X-1, pGEX-6p-1, pGEX-6p-2, pGEX-6p-3 |
| Tn3.2 | 7–1216 | B, C | 116 221 | pGEM-1 pGEM-2, pGEM-4, pGEM-Luc, pUC12, pUC13, pUC18, pUC19, pUC21, pUC57, pUC118, pUC119, pUCX |
| Tn3.3 | 77–1216 | B | 80 658 | pET20b(+), pET21a-d(+), pET22(+), pET23a-d(+), pET25b(+), pET31b(+), pET32a-c(+), pET32 Ek/LIC, pET32 Xa/LIC, |
| Tn3.4 | 76–1216 | B, C | 77 342 | pGEM-5, pGEM-5Zf(+), pGEM-T, pGEM-T easy vector, pGEMT-3P2A, pGEMT-PTE2A |
| Tn3.5 | 6–1216 | B, C | 27 508 | pQE9, pQE16, pQE30, pQE31, pQE32, pQE40, pQE60, pQE70, pQE80-L, pQE81-L. pQE82-L |
| Tn3.6 | 7–1216 | B, C, D | 8930 | pGEM-3Z, pGEM-4Z |
| Tn3.7 | 111–1171 | B, C, O, P | 6688 | pETduet-1, pET43 Ek/LIC, pET43.1a(+), pET44a-c(+), pET45b(+), pET46 Ek/LIC, pET51b(+), pET51 Ek/LIC, pET52(+), pET52 Ek_LIC |
| Tn3.8 | 7–1216 | C to M | 5750 | pGEM-3Zf(+), pGEM-3Zf(−), pGEM-11zf(+), pGEM-11Zf(−), pGEMEX-1, pGEMEX-2 |
| Tn3.9 | 77–1216 | C to M | 3920 | pGEM-7Zf(+), pGEM-7Zf(−) |
| Tn3.10 | 116–1174 | C | 2930 | pBAD24 |
| Tn3.11 | 116–1174 | 2758 | pBAD18, pBAD30, pBAD-bHS, pBAD-EGFP | |
| Tn3.12 | 116–1216 | C | 1601 | pBAD7HisB-iRFP670, pBAD/HisD-TagRFP675, pBAD/Myc-HisA, pBAD/Myc-HisB, pBAD/Myc-HisC, pBAD/gii A, pBAD/gii-B, pBAD/gii-C, pBAD/gii/Calmod, pBAD/HisB |
| Tn3.13 | 77–1216 | B, C | 270 | pGEM-5Zf(−), pGEM-9Zf(−) |
| Tn3.14 | 87–1216 | B | 167 | pET303-CT-His-Rac Kinase, pET303-CT-His |
| Tn3.15 | 13–1216 | B, C, N | 104 | pUCsg-RNA, pUCC001 |
| Tn3.16 | 113–1216 | B | 64 | pBAD-DEST49, pBAD/Myc-His/LacZ, pBAD/D-TOPO |
| Tn3.17 | 113–1161 | O | 23 | pBAD18s |
A: 5′TTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGCAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCC-3′.
B: G244 to A mutation in bla (V82 to I).
C: C545 to T mutation in bla (A182 to V); deletion of the PstI site.
D: G553 to C mutation in bla (A185 to P).
E: G226 to C, G227 to A, C228 to T mutations in bla (G76 to H).
F: G229 to A, G231 to A mutations in bla (A77 to T).
G: G232 to C mutation in bla (V78 to L).
H: G244 to A mutation in bla (G82 to H).
I: C275 to G mutation in bla.
J: A276 to G mutation in bla.
K: T277 to C mutation in bla (I93 to A).
L: A278 to G mutation in bla (I93 to A).
M: C281 to G mutation in bla (H94 to R).
N: G717 to T mutation in bla.
O: Nucleotide mutation in 5′UTR (−20 A to C).
P: Nucleotide mutation in 5′UTR (−93 A to C).
Q: Length relative to Tn3.1.
R: Nucleotide sequence of the Tn3.1 fragment is indicated by a B–P. Nucleotide changes from the Tn3.1 fragment are indicated by another letter. Numbering as depicted in Figure 1A, where the A of the AUG start codon for bla is denoted as +1.
S: Obtained from Google Scholar.