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Amplification of C. trachomatis cryptic plasmid by anchored SDA. Anchored SDA was performed with increasing amounts of template DNA input to determine the sensitivity of the system. Representative fluorescent images of each titration reaction are made after reporter hybridization and washing. Gray bars show SDA primers; white bars show mutated SDA primers for control of template background binding (noncleavable; bars are barely visible above the x axis).
