FIG. 4.

E. coli and Salmonella bacterial identification using reporter-specific and allele-specific SDA primers. A duplex anchored SDA was performed to identify E. coli and Salmonella genomic DNA samples after amplification. Amplification primers for parC and gyrA amplifications were addressed to separate locations on the microchip array. Approximately 100 pg of E. coli and Salmonella genomic DNA was addressed to all primer sets and hybridized with reporters for both E. coli gyrA and parC and Salmonella gyrA amplicons. Reporter signals remaining after application of thermal stringency were quantified to determine the genotype of the hybridized genomic DNA sample (graph). N/C, noncleavable.