TABLE 1.
Bacterial anchored SDA primersa
| Gene | Designation | Function | Sequence |
|---|---|---|---|
| Factor V | 1933 | Amplification | B-ACCGCATCGAATGCATGTCCTCGGGTCTCTGGGCTAATAGGA |
| 1934 | Amplification | B-ACGATTCAGCTCCAGACTTCTCGGGTCAGAATTTCTCAAAGG | |
| 975 | Bumper primer | ACTACAGTGACGTGGACATC | |
| 976 | Bumper primer | TGTTATCACACTGGTGCTAA | |
| 1935 | Reporter | BTR-ACTTCTAATCTGTAAGAGCAG | |
| Chlamydia | 1937 | Amplification | B-ACCGCATCGAATGCATGTCCTCGGGTACAACATCAACACCTG |
| 1938 | Amplification | B-CGATTCAGCTCCAGACTTCTCGGGTGAGACTGTTAAAGATA | |
| 1939 | Noncleavable | B-ACCGCATCGAATGCATGTCCTCCGGTACAACATCAACACCTG | |
| 1940 | Noncleavable | B-CGATTCAGCTCCAGACTTCTCCGGTGAGACTGTTAAAGATA | |
| BL1 | Bumper primer | CAGCAAATAATCCTTGG | |
| BL2 | Bumper primer | CATTGGTTGATGGATTATT | |
| 1941 | Reporter | BTR-GTCGCAGCCAAAATG | |
| 1942 | Reporter | BTR-TTCCATCAGAAGCTGT | |
| mecA | 3365 | Amplification | B-GCAAACTTAATTGGCAAATCCGCTCGGGCAGAACTCA |
| 3366 | Amplification | B-ACATCTTTAACATTAATAGCCATCCTCGGGTTTGGATTATC | |
| 3370 | Noncleavable | B-GCAAACTTAATTGGCAAATCCGCTCCGGCAGAACTCA | |
| 3371 | Noncleavable | B-ACATCTTTAACATTAATAGCCATCCTCCGGTTTGGATTATC | |
| 3367 | Bumper primer | TAAAGAAGATATTTATAGATC | |
| 3368 | Bumper primer | GGCATTGTAGCTAGCCATTCC | |
| 3369 | Reporter | Cy3-GGTTTATATCATATG | |
| Campylobacter | 3328 | Amplification | B-AAAGTAGAACAGATTTTGTCAAATCTCGGGGTATAGTGG |
| 3329 | Amplification | B-ACTTGGATATCTCATAGAAAAATCTCGGGCCATTCTAAC | |
| 3335 | Noncleavable | B-AAAGTAGAACAGATTTTGTCAAATCTCCGGGTATAGTGG | |
| 3336 | Noncleavable | B-ACTTGGATATCTCATAGAAAAATCTCCGGCCATTCTAAC | |
| 3332 | Bumper primer | TTTTATATGCTATGCAAAAT | |
| 3333 | Bumper primer | GATCCAAAGTTGCCTTGTCC | |
| 3334 | Stabilizer | GTCGTTATCACCCACATGGAGATA | |
| 3330 | Reporter | Cy3-CAGCAGTTTAT | |
| 3331 | Reporter | Cy3-TAGCAGTTTAT | |
| E. coli parC | 2764 | Amplification | B-TCGCATTAACCTCAATATGATCTCGGGGGATGGTCG |
| 2765 | Amplification | B-TCTCCAGACGATAGTTCAGTCGCTCGGGCACGGTATC | |
| 2768 | Noncleavable | B-TCGCATTAACCTCAATATGATCTCCGGGGATGGTCG | |
| 2769 | Noncleavable | B-TCTCCAGACGATAGTTCAGTCGCTCCGGCACGGTATC | |
| 2766 | Bumper primer | CACCGATCTGGAAAAGAGCTA | |
| 2767 | Bumper primer | CTTCGAGGATATGCAGGCGC | |
| 3321 | Reporter | Cy3-ATTGCCAGAGG | |
| gyrA | 3303 | Amplification | B-TGACTGGAACAAAGCCTATAAAAAATCTCGGGGTGTCGTT |
| 3304 | Amplification | B-CAGCGAGAATGGCTGCGCCATACGCTCGGGCGTGTCATA | |
| 3311 | Noncleavable | B-TGACTGGAACAAAGCCTATAAAAAATCTCCGGGTGTCGTT | |
| 3312 | Noncleavable | B-CAGCGAGAATGGCTGCGCCATACGCTCCGGCGTGTCATA | |
| 3306 | Bumper primer | TACTTTACGCCATGAACGTA | |
| 3307 | Bumper primer | GAACCGAAGTTACCCTGACC | |
| 3310 | Stabilizer | TGGGGATGGTATTTACCGATTACGT | |
| 3308 | Reporter | Cy3-CCGAGTCACCA | |
| 3309 | Reporter | Cy5-CGGAATCGCCG |
a
B, 5′ end labeled with biotin; BTR, 5′ end labeled with BTR; Cy3, 5′ end labeled with Cy3 fluorophore; Cy5, 5′ end labeled with Cy5 fluorophore.