TABLE 2.

Sensitivity of anchored SDA and cross-reactivity with other bacterial genomic DNAs^a

SDA primer set	No. of copies needed for amplification of DNA from:
	E. coli	Typhi	C. jejuni	MRSA	C. trachomatis	P. hemo	Enterococcus faecalis	Bacteroides fragilis	Kebsiella oxytoca	Yersinia enterocolitica	Shigella sonnei	Plesiomonas shigelloides	Pseudomonas aeruginosa	S. aureus	Vibrio parahaemolyticus
E. coli or Salmonella gyrA	100	100	—	—	—	—	—	—	—	—	—	—	—	—	—
Campylobacter gyrA	—	—	100	—	—	—	—	—	—	—	—	—	—	—	—
E. coli parC	10,000	—	—	—	—	—	—	—	—	—	—	—	—	—	—
mecA	—	—	—	1,000	—	—	—	—	—	—	—	—	—	—	—
Chlamydia cryptic plasmid	—	—	—	—	1,000	—	—	—	—	—	—	—	—	—	—

^aTitration experiments were performed for each anchored SDA system, and images were quantified to determine the lower limit of amplification sensitivity. The number of copies necessary for consistent amplification of template DNA is indicated. Genomic DNA from other bacterial species (100 ng) was also tested with every system to determine the degree of cross-reactivity. Typhi, Salmonella enterica, serovar Typhi; MRSA, methicillin-resistant S. aureus. —, no amplification.