Table 2.

The exploration of five class of active substances in W. pigra, H. medicinalis and H. robusta

Modes of action	Bioactive molecules	Gene copy (H. robusta)	Gene copy (W. pigra)	Gene copy (H. medicinalis)
Analgesic and anti-inflammatory effect	Antistasin	6	9	5
	Hirustasin	0	18	7
	Ghilanten	9	10	7
Extracellular matrix degradation	Hyaluronidase	1	4	5
Inhibition of platelet function	Saratin	1	4	1
Anticoagulant effect	hirudin	0	2	3
	Factor Xa inhibitor	2	1	1
	Therostasin	5	4	2
	Destabilase I a	0	5	2
Antimicrobial effect	Destabilase I a	0	5	2
Total		24	57	33

^a Destabilase I, involved in anticoagulant effect and antimicrobial effect