Puff durations were either 1, 2, or 4.3 seconds, while puff volume was either 13, 26, 56, 40, or 80 mL. The x-axis shows the fluid and aerosol samples. Flavor chemicals are listed on the left y-axis, and concentrations in mg/mL are shown on the color scale to the right. The numbers at the top of each column give the concentration (mg/mL) of all flavor chemicals plus nicotine in each sample.