Fig. 5.

Hypofractionated RT regimen improves median survival and inhibits tumor proliferation in syngeneic GB models. a Schematic representation of the in vivo fractionated RT regimens with and without TMZ. b Kaplan–Meier plots show median survival of N = 6 mice per treatment group. c Micrographs of H&E and immunohistochemical staining for Ki67 + proliferation, γH2A.X DNA damage, and CD45 + immune cell infiltration. H&E scale bar = 5 mm, and histological micrographs = 200 µm. d Column graphs show the mean number of positive cells ± SEM per high power field. Five high power field were averaged per mouse. ^ap < 0.05 vs untreated, ^bp < 0.05 vs TMZ, ^cp < 0.05 vs C-RT, ^dp < 0.05 vs H-RT, ^ep < 0.05 vs TMZ/C-RT, and ^fp < 0.05 vs TMZ/H-RT determined by on-way ANOVA with Tukey’s multiple comparison test. nt, no discernible tumor