Table 2.

Primers, probes and standards used in this study.

Primer/probe	Sequence	Source
2019-nCoV_N1 F	5′-GACCCCAAAATCAGCGAAAT-3′	(US Centers for Disease Control and Prevention, 2019)
2019-nCoV_N1 R	5′-TCTGGTTACTGCCAGTTGAATCTG-3′	(US Centers for Disease Control and Prevention, 2019)
2019-nCoV_N1 Probe	5′-FAM-ACCCCGCATTACGTTTGGTGGACC-MGBNFQ-3′	(US Centers for Disease Control and Prevention, 2019)
PMMV F	5′-GAGTGGTTTGACCTTAACGTTGA-3′	(Haramoto et al., 2013)
PMMV R	5′-TTGTCGGTTGCAATGCAAGT-3′	(Haramoto et al., 2013)
PMMV Probe	5′-6FAM-CCTACCGAAGCAAATG-MGBNFQ-3′	(Zhang et al., 2005)
HCoV-229E F	5′-TTCCGACGTGCTCGAACTTT-3′	Lilly Pang (APL) AB
HCoV-229ER	5′-CCAACACGGTTGTGACAGTGA-3′	Lilly Pang (APL) AB
HCoV-229 E Probe	5′-FAM-TCCTGAGGTCAATGCA-MGBNFQ-3′	Lilly Pang (APL) AB
HCoV-229E G-block	5′-GTGTACCTGAAACAAAACCCATTGTAATTTTTCCGACGTGCTCGAACTTTTTTTCCTGAGG-3′	Lilly Pang (APL) AB
N1 Standard	Custom IDT Twist RNA Control 2	Twist Bioscience
PMMoV Standard	RNA N gene standard in vitro transcribed from an IDT gBlock DNA	IDT