Table 1.
The viral titers of viruses before and after 5-generation's serial passaging
| Rescued viruses | Parent Virus HA titer (log2) | Parent Virus (generation 0) (log10 copies/ml)a | 5th Generation from MDCK cell line (log10 copies/ml) | 5th Generation from Mouse lung (log10 copies/ml) |
|---|---|---|---|---|
| A2093HANAPB2/WSN | 10 | 8.05 ± 0.02 | 2.16 ± 0.17 | 2.56 ± 0.82 |
| A2093HANAPB1/WSN | 6 | 7.09 ± 0.04 | 7.59 ± 0.01 | 6.59 ± 0.48 |
| A2093HANAPA/WSN | 10 | 8.33 ± 0.06 | 5.48 ± 0.00 | 7.31 ± 0.28 |
| A2093HANANP/WSN | 9 | 8.06 ± 0.20 | 2.33 ± 0.05 | 6.73 ± 0.27 |
| A2093 | 11 | 7.76 ± 0.02 | 6.60 ± 0.00 | 6.16 ± 0.03 |
| WSN | 7 | 7.87 ± 0.04 | 6.74 ± 0.00 | 7.86 ± 0.30 |
aQ-PCR were repeated twice, mean and sd were calculated to estimate the viral particles. It was suggested that copy number of viral particles were 20–60 times of one infectious virus[54]