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Michaelis–Menten kinetics of human plasmin in the presence of NSGM 2 (A) or its polyphenolic precursor 1j (B). The initial rate of hydrolysis at various substrate concentrations was measured spectrophotometrically in pH 7.4 buffer at 37 °C. Solid lines represent the nonlinear regressional fits to the data using the eq 2 to yield KM and VMAX.
