Direct inhibition of serine proteases by NSGM 2. The inhibition of plasmin (●), thrombin (♦), trypsin (■), factor XIa (□), chymotrypsin (○), and factor Xa (△) by inhibitor 2 was studied using chromogenic substrate hydrolysis assay at pH 7.4 and 37 °C, as described in Experimental Part. Solid lines represent the sigmoidal dose–response fits (eq 1) to the data to obtain the values of IC50, ΔY, and HS.