Figure 6.

Inhibition of human plasmin by NSGM 2 as a function of NaCl and/or UFH: (A) with NaCl and without UFH; (B) without NaCl and with UFH; (C) with both NaCl and UFH. Inhibition was studied using the chromogenic substrate hydrolysis assay at pH 7.4 and 37 °C. (D) Profile of recovery of plasmin activity in the presence of increasing concentrations of protamine (0–4 mg/mL) in the presence and absence of NSGM 2 (200 μM). Solid lines represent the sigmoidal fits to the data to obtain IC₅₀ (or EC₅₀), HS, Y_M, and Y₀ using eq 1 or 4, as described in the Experimental Part.