Table 2.

Michaelis–Menten Kinetics of Human Plasmin in the Presence of NSGM 2 or Its Precursor 1j^a

	[inhibitor] (μM)	KM b (mM)	VMAX b (mAU/min)
2	0	0.050 ± 0.001	42.2 ± 2.3
	1.6	0.050 ± 0.001	36.3 ± 2.0
	8	0.020 ± 0.001	29.0 ± 0.6
	40	0.020 ± 0.007	22.1 ± 1.9
	100	0.008 ± 0.003	15.1 ± 1.0
	200	0.004 ± 0.001	10.4 ± 0.5
1j	0	0.083 ± 0.014	50.6 ± 2.4
	100	0.357 ± 0.082	51.0 ± 4.6
	240	0.807 ± 0.180	51.7 ± 3.9
	400	1.122 ± 0.233	51.3 ± 4.0

^aK_M and V_MAX values of Spectrozyme PL substrate hydrolysis by human plasmin were measured under physiologic conditions. mAU indicates milliabsorbance units.

^bError represents ±1 SE.