Table 3.

Selectivity of NSGM 2 and Its Precursor 1j against a Panel of Related Serine Proteases^a

inhibitor	enzyme	IC50 b (μM)	ΔY b,c (%)	SId
2	plasmin	7.2 ± 0.6	98 ± 5
	thrombin	156 ± 14	77 ± 8	22
	FXa	~505	ND	70
	FXIa	180 ± 20	93 ± 11	26
	FIXa	>2000	ND	>277
	FXIIa	>1000	ND	>138
	trypsin	>440	ND	>61
	chymotrypsin	196 ± 39	92 ± 9	27
1j	plasmin	116 ± 9	111 ± 8
	thrombin	267 ± 28	91 ± 11	2
	FXa	212 ± 30	104 ± 17	2
	FXIa	208 ± 32	109 ± 16	2

^aDirect inhibition of human enzymes was measured in appropriate Tris-HCl buffers of pH 7.4 at 37 °C. Inhibition was monitored spectrophotometrically. See Experimental Part for details.

^bSelectivity index.

^cErrors represent ±1 SE.

^dNot determined.