Table 2.
Biophysical properties of cetuximab-derived scFv-monoFc proteins.
| Version | Titer (µg/mL) | aSEC %POI | DLS Temp (°C) | EGFR KD (nM) | ka [1/(M·s)] × 105 | kd (1/s) × 10−3 |
|---|---|---|---|---|---|---|
| Cetuximab | 163 ± 39 | 93.6 ± 2.6 | 47.2 ± 1.0 | 3.18 ± 0.18 | 4.44 ± 1.13 | 1.42 ± 0.44 |
| N85E | 116 ± 6 | 94.9 ± 3.1 | 44.5 ± 1.3 | 3.16 ± 0.35 | 4.44 ± 0.91 | 1.42 ± 0.44 |
| H1 | 309 ± 26 | 91.5 ± 7.1 | 46.2 ± 0.9 | 13.1 ± 1.2 | 7.18 ± 1.68 | 9.27 ± 1.34 |
| H2 | 308 ± 3 | 83.7 ± 1.9 | 52.6 ± 1.3 | 6.77 ± 2.10 | 6.65 ± 0.73 | 4.58 ± 1.89 |
| H3 | 376 ± 12 | 88.6 ± 2.4 | 53.0 ± 1.3 | 6.74 ± 1.61 | 6.61 ± 1.11 | 4.54 ± 1.81 |
| H4 | 220 ± 3 | 92.2 ± 0.0 | 51.3 ± 1.1 | 6.79 ± 1.35 | 5.71 ± 0.78 | 3.93 ± 1.30 |
| H5 | 211 ± 22 | 97.4 ± 0.9 | 48.7 ± 1.3 | 6.92 ± 1.58 | 5.63 ± 0.67 | 3.95 ± 1.35 |
| H6 | 411 ± 59 | 86.8 ± 2.1 | 52.4 ± 2.1 | 3.08 ± 0.68 | 4.81 ± 0.91 | 1.51 ± 0.61 |
| H7 | 418 ± 8 | 88.1 ± 2.7 | 52.3 ± 2.0 | 3.45 ± 0.81 | 5.04 ± 0.92 | 1.77 ± 0.73 |
| H8 | 412 ± 5 | 82.6 ± 5.5 | 49.7 ± 1.4 | 3.05 ± 0.62 | 4.50 ± 0.54 | 1.39 ± 0.44 |
| H9 | 506 ± 14 | 92.7 ± 2.3 | 50.5 ± 2.4 | 3.14 ± 0.53 | 4.47 ± 0.97 | 1.43 ± 0.54 |
| H10 | 394 ± 23 | 85.6 ± 6.2 | 50.2 ± 1.5 | 2.27 ± 0.38 | 4.74 ± 1.31 | 1.10 ± 0.48 |
| H11 | 372 ± 73 | 88.0 ± 3.4 | 52.2 ± 2.0 | 3.42 ± 0.36 | 4.76 ± 1.33 | 1.65 ± 0.62 |
Values are the average and standard deviation of two independent experiments.