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Biallelic mutations affect SPATC1L expression. (a) Immunofluorescence staining of SPATC1L (red) in the testis from a man with obstructive azoospermia. Scale bars = 5 μm. (b) Western blot analysis of the protein expression of Flag-vector, Flag-SPATC1L, and Flag-SPATC1Lp.Arg304Cys, and Flag-SPATC1Lp.Glu332X. β-Tubulin was used as the loading control. SPATC1L: spermatogenesis and centriole-associated 1 like; DDX4: DEAD-box helicase 4; DAPI: 4’,6-diamidino-2-phenylindole.
