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Characterization of proteins, polysaccharides, and protein-polysaccharide conjugates by HPLC. HPLC characterization of CPS4 (A), PspA4-SA (B), PspA4-SA-Bio-CPS4 (C), PsaA-PspA23-SA (D), and PsaA-PspA23-SA-Bio-CPS4 (E) at OD280 and OD206. The maximum absorption peak was observed at OD280 for proteins and OD206 for polysaccharides.
