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Characterization of detoxification enzyme ADH3 and mainstream commercial enzyme CPA on OTA degradation. (A) The amino acid sequence of ADH3 (the red and amplified residues are the active sites). (B) The purified recombinant ADH3 overexpressed by E. coli BL21. (C) The effects of metal-chelator and protein denaturant on degradation activity. (D) The metal ion effect on degradation activity. (E) The temperature evaluation and optimal temperature. (F) The pH evaluation and optimal pH.
