Table 1.
Strains and Plasmid Used in This Study
| Strains | Relevant characteristics | References |
|---|---|---|
| Escherichia coli AB2834 | K12 ∆aroE | Yale University |
| E. coli Inha103 | AB2834 ∆tyrR ∆ptsG ∆pykA ∆lacI:: Plac_aroB_aroD_Plac_aroG_aroF_Plac_ppsA_galP | (Choi et al., 2019) |
| Inha 201 | AB2834 ∆aroL ∆aroK | This study |
| Inha 202 | AB2834 ∆tyrR ∆ptsG ∆pykA ∆aroL ∆aroK | This study |
| Inha 203 | AB2834 ∆tyrR ∆ptsG ∆pykA ∆lacI:: Plac_aroB_aroD_Plac_aroG_aroF_Plac_ppsA_galP ∆aroL ∆aroK | This study |
| Inha 204 | AB2834 ∆tyrR ∆ptsG ∆pykA ∆lacI:: Plac_aroB_aroD_Plac_aroG_aroF_Plac_ppsA_galP ∆aroL ∆aroK ∆ydiB | This study |
| Inha 205 | AB2834 ∆tyrR ∆ptsG ∆pykA ∆lacI:: Plac_aroB_aroD_Plac_aroG_aroF_Plac_ppsA_galP ∆aroL ∆aroK ∆shiA | This study |
| Inha 206 | AB2834 ∆tyrR ∆ptsG ∆pykA ∆lacI:: Plac_aroB_aroD_Plac_aroG_aroF_Plac_ppsA_galP ∆aroL ∆aroK ∆ydiN | This study |
| Inha 207 | AB2834 ∆tyrR ∆ptsG ∆pykA ∆lacI:: Plac_aroB_aroD_Plac_aroG_aroF_Plac_ppsA_galP ∆aroL ∆aroK ∆shiA ∆ydiN | This study |
| Inha 208 | AB2834 ∆tyrR ∆ptsG ∆pykA ∆lacI:: Plac_aroB_aroD_Plac_aroG_aroF_Plac_ppsA_galP ∆aroL ∆aroK ∆shiA ∆pta-ackA | This study |
| Inha 209 | AB2834 ∆tyrR ∆ptsG ∆pykA ∆lacI:: Plac_aroB_aroD_Plac_aroG_aroF_Plac_ppsA_galP ∆aroL ∆aroK ∆shiA ∆aroEAB2834::aroEK12 | This study |
| Inha 211 | Inha 201/PoppA-aroE | This study |
| Inha 212 | Inha 202/PoppA-aroE | This study |
| Inha 213 | Inha 203/PoppA-aroE | This study |
| Inha 214 | Inha 204/PoppA-aroE | This study |
| Inha 215 | Inha 205/PoppA-aroE | This study |
| Inha 216 | Inha 206/PoppA-aroE | This study |
| Inha 217 | Inha 203/pPoppA-aroE-ydiB | This study |
| Inha 218 | Inha 203/PoppA-aroE-tktA | This study |
| Inha 219 | Inha 205/PoppA-aroE-tktA | This study |
| Inha 220 | Inha 206/PoppA-aroE-tktA | This study |
| Inha 221 | Inha 207/PoppA-aroE-tktA | This study |
| Inha 222 | Inha 208/PoppA-aroE-tktA | This study |
| Inha 223 | Inha 209/PoppA-aroE | This study |
| Inha 224 | Inha 209/PoppA-aroE-tktA | This study |
| Plasmids | Relevant characteristics | References |
| pMESK4 | pUC18 modification vector including oppA promoter asbFEopt- aroYEopt- catAEopt | 2019, |
| pPoppA-aroE | pMESK4 modification vector including oppA promoter & aroEK12 | This study |
| pPoppA-aroE-ydiB | pMESK1 modification vector including oppA promoter & aroEK12& ydiB | This study |
| pPoppA-aroE-tktA | pMESK1 modification vector including oppA promoter & aroEK12 & tktA | This study |
| pCas | repA101(Ts) kan Pcas-cas9 ParaB-Red lacIq Ptrc-sgRNA-pMB1 | Addgene, (Jiang et al., 2015) |
| pTargetF | pMB1 aadA sgRNA-cadA | Addgene, (Jiang et al., 2015) |
| pTarget-ydiB | pTargetF containing sgRNA of ydiB and its homologous arms | This study |
| pTarget-shiA | pTargetF containing sgRNA of shiA and its homologous arms | This study |
| pTarget-ydiN | pTargetF containing sgRNA of ydiN and its homologous arms | This study |
| pTarget-pta | pTargetF containing sgRNA of pta-ackA and its homologous arms | This study |
| pTarget-aroE | pTargetF containing sgRNA of aroE and its homologous arms | This study |
| pTarget-aroE-ins | pTargetF containing sgRNA of disrupted aroE fragment and aroEK12gene | This study |
| pKOV | The suicide vector containing the Bacillus subtilis sacB gene and temperature sensitive pSC101 replication origin | Addgene |
| pKOV-aroK | pKOV containing a PCR fragment for disruption of the aroK gene | This study |
| pKOV-aroL | pKOV containing a PCR fragment for disruption of the aroL gene | This study |