FIG 1.

Algal and bacterial growth during cocultivation. For each time point, four replicate cocultivation flasks were sampled for growth monitoring. (a and b) Algal growth was determined using three technical replicates per coculture (a), and bacterial growth was measured using technical duplicates per coculture (b). The data are presented as the means of the technical replicates. Error bars indicate the standard deviations. RNA was extracted from three cocultures at four time points during the algal-bacterial interaction (orange). A pool of RNA extracts from all four time points was used for PacBio Iso-Seq sequencing, while individual Illumina RNA sequencing libraries were prepared for each of the 12 RNA samples to allow time-resolved differential gene expression analyses.