FIG 2.

Complementary long- and short-read sequencing of E. huxleyi CCMP3266 resulted in a hybrid transcriptome with improved transcript length, and full-length transcript isoform recovery. (a) PacBio long reads (LR) and Illumina total RNA short reads (SR) were used to construct a hybrid transcriptome, and two nonhybrid LR and SR control transcriptomes. A third nonhybrid E. huxleyi CCMP371 control transcriptome was downloaded from the Transcriptome Shotgun Assembly (TSA) database (31). (b) The quality of the hybrid transcriptome was evaluated by comparing it to the three nonhybrid control transcriptomes.