B. pseudocatenulatum growth and glycoprofiling on human milk oligosaccharides (HMOs). B. pseudocatenulatum isolates grown on 2% (wt/vol) pooled HMOs with strong (A) and weak (B) growth phenotypes. B. longum subsp. infantis ATCC 15697 and B. animalis subsp. lactis ATCC 27536 were included as positive and negative controls, respectively. Mean optical density at 600 nm of two independent biological replicates. (C) Glycoprofiling of the dominant HMOs from this HMO pool consumed by B. pseudocatenulatum isolates grown on 2% (wt/vol) HMOs. Percent consumption was calculated as the difference in HMO structure abundance at 96 h relative to 0 h. 2′-FL, 2′-fucosyllactose; LDFT, lactodifucotetraose; LNT, lacto-N-tetraose; LNnT, lacto-N-neotetraose; LNFP I, II, and III, lacto-N-fucopentaose type I, II, and III; LNDFH I and II, lacto-N-difucohexaose type I and II.