TABLE 3.

Expression fold changes of fucosylated HMO utilization cluster, LNB/GNB cluster, and other HMO-utilizing genes in B. pseudocatenulatum MP80 strain during growth in 2′-FL and LNFP1^a

Gene ID	Annotated function	Fold change during growth on substrates
		2′-FL	LNFPI
FHMO utilization cluster
2765237614	1,2-α-l-fucosidases (GH95)	21.71	16.28
2765237615	α-1,3/1,4-l-fucosidase (GH29)	22.24	16.07
2765237616	l-fucose mutarotase	20.35	15.08
2765237617	4-Hydroxy-tetrahydrodipicolinate synthase	26.74	21.77
2765237618	Amido hydrolase	42.09	48.53
2765237619	l-fucose dehydrogenase	40.28	51.69
2765237620	l-fuconate dehydratase	40.34	45.18
2765237621	Solute-binding protein	30.82	38.22
2765237622	ABC permease	28.41	38.05
2765237623	ABC permease	29.60	41.63
2765237623	Transcriptional regulator	2.58	2.61
LNB/GNB cluster
2765237505	N-acetylglucosamine-6-phosphate deacetylase	1.03	66.36
2765237506	Glucosamine-6-phosphate deaminase	0.60	48.50
2765237507	β-N-acetylhexosaminidase	0.60	21.57
2765237508	Predicted NBD/HSP70 family sugar kinase	0.51	31.28
2765237509	ABC permease	0.60	12.39
2765237510	ABC permease	0.48	7.91
2765237511	Type 1 HMO solute-binding protein	0.61	2.17
Other important HMO-utilizing genes
2765236220	β-Galactosidase	0.97	12.04
2765237192	β-Galactosidase	1.92	6.34
2765237343	β-Galactosidase	2.02	1.11
2765237514	β-Galactosidase	0.67	0.38
2765237579	β-Galactosidase	1.36	1.75
2765237612	β-Galactosidase	1.92	2.28
2765236421	β-N-acetylhexosaminidase	1.97	0.84

^aLevel of expression is shown as fold change compared to the lactose control. Fold change values in bold have significant FDR P values (P ≤ 0.05).