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Correlation of kan Gene Activation with One Base Substitution at the -10 Promoter Region
| Streptomyces griseus | ||
|---|---|---|
| SS-1198 | SS-1198PR | |
| Gene | kan0 | Kan |
| -10 promoter sequence | CATGAT | TATGAT |
| Promoter activity | Weak | Strong |
| mRNA level | Low | High |
| AAC(3) level | Low | High |
| KM resistance | <5 μg/ml | 1000 μg/ml |
