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. 2021 Dec 1;3(1):zqab064. doi: 10.1093/function/zqab064

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© The Author(s) 2021. Published by Oxford University Press on behalf of American Physiological Society.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Analysis of GCaMP6s fluorescence changes in bystander keratinocytes at increasing distance from the photodamage site. (A) In each panel, the vertical black dash-dotted line at 0 s marks the end of the 0.5 s photodamage time interval. Data are mean (solid line) ± s.e.m. (dashed lines) vs. time for n = 60 experiments in 15 mice. (B) Amplitude (a_20s) of the signal at time t = 20 s. (C) Area (I) under the trace, computed between 0 and 20 s. (D) Slope (s) of the trace at the inflection point (mean ± s.e.m). Data in (B-D) are mean ± s.e.m. vs. bystander cell distance from the photodamage site.

Inline graphic — Analysis of GCaMP6s fluorescence changes in bystander keratinocytes at increasing distance from the photodamage site. (A) In each panel, the vertical black dash-dotted line at 0 s marks the end of the 0.5 s photodamage time interval. Data are mean (solid line) ± s.e.m. (dashed lines) vs. time for n = 60 experiments in 15 mice. (B) Amplitude (a_20s) of the signal at time t = 20 s. (C) Area (I) under the trace, computed between 0 and 20 s. (D) Slope (s) of the trace at the inflection point (mean ± s.e.m). Data in (B-D) are mean ± s.e.m. vs. bystander cell distance from the photodamage site.