Fig. 2.
Establishment of a minimal cassette for high-level mevalonate production. (A) Comparison between the mevalonate pathways from Saccharomyces cerevisiae and Enterococcus faecalis. YLG6 is a negative control containing an empty 2μ plasmid. The average mevalonate production of six independent colonies, following a 2-day high cell-density fermentation. Error bars represent one standard deviation. (B) The E. faecalis mevalonate pathway expressed alone or with the inhibition-insensitive Se-acsL641P in 2μ plasmids, in wild-type or Δadh1/Δgpd1 deletion backgrounds. (C) Strains with mevalonate cassette integrated in δ-sites. B and C show the average mevalonate production of 7-day fermentations starting from low cell density. Error bars represent one standard deviation from three biologically independent replicates. ***P < 0.001.