Fig. 6.

Mevalonate production using strain JCY87 in 2 L fed-batch bioreactors operated initially in 20 g/l glucose, 15 μM pantothenate, and 2 mM methionine. At 14 h, a 4.5 ml/h feed was started containing 40% glucose in twofold concentrated SC-Ura media containing 15 μM pantothenate and 50 mM methionine. (A) Mevalonate and ethanol production. (B) Glucose concentration and cell density in the bioreactor. The average values of three independent bioreactor experiments, with error bars representing the standard deviation. See methods for complete details.