Table 1.
Plasmids Used in This Study
| Plasmid Name | Description | Source |
|---|---|---|
| pVSLS19 | 2μ plasmid, URA3 marker, PGPD1-Ef-mvaE-TADH1 PTEF1-Ef-mvaS-TACT1 | This study |
| pVSLS22 | 2μ plasmid, URA3 marker, PGPD1-Ef-mvaE-TADH1 PTEF1-Ef-mvaS-TACT1 PPGK1-Se-acsL641P-TCYC1 | This study |
| pVSLS32 | 2μ plasmid, URA3 marker, PGPD1-Bp-xfp-TADH1 PPGK1-Ec-ackA-TCYC1 | This study |
| JMC_P4 | 2μ plasmid, TRP1 marker, PGPD1-Bp-xfp-TADH1 PPGK1-Ec-ackA-TCYC1 | This study |
| JMC_P21 | 2μ plasmid, URA3 marker, PGPD1-ALD6-HA-TADH1 | This study |
| JMC_P22 | 2μ plasmid, URA3 marker, PGPD1-Bp-xfp-TADH1 PPGK1-Bs-pta-TCYC1 | This study |
| JMC_P26 | 2μ plasmid, URA3 marker, PTPI1-CAB1-TPGK1 | This study |
| JMC_P28 | 2μ plasmid, URA3 marker, PGPD1-Bp-xfp-TADH1 PPGK1-Ec-ackA-TCYC1 PTPI1-CAB1-TPGK1 | This study |
| JMC_P39 | 2μ plasmid, URA3 marker, PGPD1-Bp-xfp-TADH1 PPGK1-Bs-pta-TCYC1 PTPI1-CAB1-TPGK1 | This study |
| Sip_L33 | 2μ plasmid, URA3 marker, PGPD1-ERG1–TADH1 PTEF1-ERG13-TACT1 (PTEF1-HMG1-TCYC1) | This study |
| p426ADH | 2μ plasmid, URA3 marker, PADH-empty -TCYC1 | (Mumberg et al., 1995) |
| p39 | 2μ plasmid, URA3 marker, PGPD1-empty-TADH1 | This study |
| pYZ23 | Plasmid containing Lox71-bleMX6-Lox66 δ-integration cassette | (Zhao et al., 2018) |
| JMC_P10 | Plasmid for δ-integration of PGPD1-Ef-mvaE-TADH1 PTEF1-Ef-mvaS-TACT1 PPGK1-Se-acsL641P-TCYC1 | This study |
| pV1382_ERG9_1 | CRISPR plasmid for replacement of endogenous PERG9 with PMET3, URA3 marker | This study |
| CRISPR_ERG9_RT_1 | CRISPR repair template for replacement of endogenous PERG9 with PMET3, HIS3 marker | This study |