Table 3.
Yeast Strains Used in This Study
| Strain | Description | Genotype | Source |
|---|---|---|---|
| CEN.PK2-1C | Wild type | MATa ura3-52 trp1-289 leu2-3_112 his3Δ1 MAL2-8C SUC2 | (Entian & Kötter, 2007) |
| JAy498 | adh1Δ gpd1Δ | CEN.PK2-1C adh1::KanMX, gpd1::HygB | This study |
| YLG6 | WT (P426ADH) | CEN.PK2-1C (P426ADH—empty vector) | This study |
| SIY2 | WT (pVSLS22) | CEN.PK2-1C (Ef-mvaE, Ef-mvaS, Se-acsL641P) | This study |
| SIY23 | WT (Sip_L33) | CEN.PK2-1C (ERG10, ERG13, tHMG1) | This study |
| JCY4 | WT (pVSLS19) | CEN.PK2-1C (Ef-mvaE, Ef-mvaS) | This study |
| JCY8 | adh1Δgpd1Δ (pVSLS19) | JAy498 (Ef-mvaE, Ef-mvaS) | This study |
| JCY9 | adh1Δgpd1Δ (pVSLS22) | JAy498 (Ef-mvaE, Ef-mvaS, Se-acsL641P) | This study |
| JCY17 | WT (JMC_P4, pVSLS22) | CEN.PK2-1C (EfMvaE, EfMvaS, SeAcsL641P) (BpXfp, EcAckA) | This study |
| JCY31 | PMET3-ERG9 (pVSLS22) | JCY26-1 (Ef-mvaE, Ef-mvaS, Se-acsL641P) | This study |
| JCY33 | adh1Δgpd1Δ PMET3-ERG9 (pVSLS22) | JCY28-1 (Ef-mvaE, Ef-mvaS, Se-acsL641P) | This study |
| JCY37 | adh1Δgpd1Δ δ-Ef-mvaE, Ef-mvaS, Se-acsL641P | JAy498 δ-Ef-mvaE, Ef-mvaS, Se-acsL641P::lox71-bleMX6-lox66 | This study |
| JCY46 | adh1Δgpd1Δ PMET3-ERG9 δ-Ef-mvaE, Ef-mvaS, Se-acsL641P | JCY28-1 δ-Ef-mvaE, Ef-mvaS, Se-acsL641P::lox71-bleMX6-lox66 | This study |
| JCY48 | PMET3-ERG9 δ Ef-mvaE, Ef-mvaS, Se-acsL641P | JCY26-1 δ-Ef-mvaE, Ef-mvaS, Se-acsL641P::lox71-bleMX6-lox66 | This study |
| JCY51 | δ-Ef-mvaE, Ef-mvaS, Se-acsL641P | CEN.PK2-1C δ-Ef-mvaE, Ef-mvaS, Se-acsL641P::lox71-bleMX6-lox66 | This study |
| JCY54 | PMET3-ERG9 δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (pVSLS32) | JCY48-A6 (Bp-xfp, Ec-ackA) | This study |
| JCY65 | PMET3-ERG9 δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (JMC_P21) | JCY48-A6 (ALD6) | This study |
| JCY67 | PMET3-ERG9 δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (JMC_P22) | JCY48-A6 (Bp-xfp, Bs-pta) | This study |
| JCY73 | PMET3-ERG9 δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (p39) | JCY48-A6 (empty vector) | This study |
| JCY87 | PMET3-ERG9 δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (JMC_P26) | JCY48-A6 (CAB1) | This study |
| JCY88 | PMET3-ERG9 δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (JMC_P28) | JCY48-A6 (Bp-xfp, Ec-ackA, CAB1) | This study |
| JCY90 | adh1Δgpd1Δ δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (p39) | JCY37-D3 (empty vector) | This study |
| JCY91 | adh1Δgpd1Δ δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (pVSLS32) | JCY37-D3 (Bp-xfp, Ec-ackA) | This study |
| JCY93 | δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (p39) | JCY51-A6 (empty vector) | This study |
| JCY94 | δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (pVSLS32) | JCY51-A6 (Bp-xfp, Ec-ackA) | This study |
| JCY98 | δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (pVSLS22) | JCY51-A6 (Ef-mvaE, Ef-mvaS, Se-acsL641P) | This study |
| JCY99 | adh1Δgpd1Δ δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (pVSLS22) | JCY37-D3 (Ef-mvaE, Ef-mvaS, Se-acsL641P) | This study |
| JCY110 | PMET3-ERG9 δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (JMC_P39) | JCY48-A6 (Bp-xfp, Bs-pta, CAB1) | This study |
| JCY112 | PMET3-ERG9 δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (pVSLS22) | JCY48-A6 (Ef-mvaE, Ef-mvaS, Se-acsL641P) | This study |
| JCY113 | adh1Δgpd1Δ δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (JMC_P22) | JCY37-D3 (Bp-xfp, Bs-pta) | This study |
| JCY115 | adh1Δgpd1Δ δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (JMC_P21) | JCY37-D3 (ALD6) | This study |
| JCY116 | adh1Δgpd1Δ δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (JMC_P26) | JCY37-D3 (CAB1) | This study |
| JCY117 | δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (JMC_P22) | JCY51-A6 (Bp-xfp, Bs-pta) | This study |
| JCY119 | δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (JMC_P21) | JCY51-A6 (ALD6) | This study |
| JCY120 | δ-Ef-mvaE, Ef-mvaS, Se-acsL641P (JMC_P26) | JCY51-A6 (CAB1) | This study |