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(a) Giemsa‐stained blood smear showing merozoites of large Babesia spp (100×). (b) Polymerase chain reaction (PCR) analysis with primers 455–479F and 793‐772R specific for 18S rRNA gene of B. canis (Lane 1), the corresponding PCR product from lane 1 was analyzed by seminested PCR using B. canis subspecies canis specific primers BCC‐F and 793‐772R (lane 2). Molecular weight marker (lane M: 50 bp) and negative (i.e., no‐DNA) control (NC)
