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. 2021 Nov 16;8(1):200–210. doi: 10.1002/vms3.669

TABLE 1.

Primers used in the present study

Target genes Primer Sequence (5′–3′) Amplicon size (bp) Annealing temperature (°C) References
ddlE. faecium

F: GCAAGGCTTCTTAGAGA

R: CATCGTGTAAGCTAACTTC

550 50 Dutka‐Malen S et al., 1995
tetA

F: GGTTCACTCGAACGACGTCA

R: CTGTCCGACAAGTTGCATGA

577 57 Randall et al., 2004
tetB

F: CCTCAGCTTCTCAACGCGTG

R: GCACCTTGCTGATGACTCTT

634 56 Randall et al., 2004
CITM

F: TGGCCAGAACTGACAGGCAAA

R: TTTCTCCTGAACGTGGCTGGC

462 47 Van et al., 2008
blaTEM

F: CATTTCCGTGTCGCCCTTAT

R: TCCATAGTTGCCTGACTCCC

793 56 Randall et al., 2004
ereA

F: GCCGGTGCTCATGAACTTGAG

R: CGACTCTATTCGATCAGAGGC

419 52 Van et al., 2008
SHV

F: TCGCCTGTGTATTATCTCCC

R: CGCAGATAAATCACCACAATG

768 52 Van et al., 2008
aadA1

F: TATCCAGCTAAGCGCGAACT

R: ATTTGCCGACTACCTTGGTC

447 58 Van et al., 2008