Figure 4.

Overexpression of miR-514-a-3p attenuates IGF-induced proliferation in the human placenta. First-trimester placental explants (n = 6) were transfected with miR-514a-3p (200 nM), miR-758-3p mimics (200 nM), non-targeting miRNA-mimic (pre-miR-control; 200 nM) or SHP-2 siRNA (100 nM). IGF-I (10 nM) or vehicle was added after 48 h, then 24 h later, cytotrophoblast proliferation was assessed by immunohistochemical analysis of BrdU incorporation and expressed as the number of immunopositive cells as a percentage of total nuclei. Data are presented as median and were analysed by Kruskal–Wallis followed by Dunn’s post hoc test. n  = 6; *P< 0.05; **P< 0.01.