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. 2021 Jul 5;50(2):601–616. doi: 10.1093/nar/gkab527

Figure 4.

Figure 4.

Re-selection of the second-generation RNA library against individual L7Ae mutants (LS1-LS20) and L7Ae. (A) Schematic illustration of the re-selection experiment. A focused RNA library containing the 1991 most abundant RNAs after PD-SELEX and 9 control RNAs was selected against individual L7Ae or its mutant immobilized on magnetic beads. (B, C) Box plots representing enrichment factors (EFs) of a subset of the RNAs that contains consensus sequence 1 (UUGUGASGC, S = C or G) (B) or consensus sequence 2 (UCCAUGACGC) (C). EF is defined as the abundance of an RNA after selection divided by the abundance before selection.