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. 2021 Jul 5;50(2):601–616. doi: 10.1093/nar/gkab527

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Binding selectivities of LS4, LS12, and L7Ae for (A) CS1 RNA and its mutants, and (B) CS2 RNA and its mutants. The bar graphs represent means and standard deviations of the K_D values derived from at least two independent experiments. Red dots represent binding selectivity (ratio of the K_D values) of the RNA for the two proteins. Asterisks indicate either no binding or weak binding with undetermined K_D value based on SPR. Binding selectivity could not be determined for these RNAs (N.D.). SPR sensorgrams and binding parameters are shown in Supplementary Figure S10 and Supplementary Table S4.