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. 2021 Jul 5;50(2):601–616. doi: 10.1093/nar/gkab527

Table 1.

Binding properties of model RNAs and proteins determined by SPR.

RNA Protein k on (M–1 s–1) k off (s–1) K D (M)
Box C/D Kt L7Ae (1.41 ± 1.11) × 107 (2.77 ± 1.75) × 10–4 (2.13 ± 0.43) × 10–11
dL7Ae N.B.
dKt L7Ae N.B.
dL7Ae N.B.

k on, koff, KD values are means and standard deviations of at least two independent experiments.

N.B.: no binding detected at the protein concentrations tested.