Table 1.

Mug20-LacI fusion is most active in creating a meiotic recombination hotspot

ade6 allele	No fusion	Rec10-LacI (rec10-233)	Mug20-LacI (mug20-231)	Rec25-LacI (rec25-230)	Rec27-LacI (rec27-232)
3101	89 ± 13 (8)	209 + 28 (5)	529 ± 27 (8)	60 (1)*	158 ± 27 (5)

Intragenic recombinant frequency (Ade⁺ per 10⁶ viable spores) was assayed with ade6-3101 and ade6-52 in either the absence or presence of different LinE-LacI fusion proteins. ade6-3101 contains 8 copies of the lacO operator substituted for part of the ade6 gene (Figure 1). Data are mean ± SEM from (n) crosses. See Table S5 for data with additional ade6::lacO alleles.

*In independent experiments, the frequency was 70 ± 5 (n = 6) with Rec25-LacI and 119 ± 10 (n = 8) with Rec25.