Table 3.

LacI fusion to the N-terminus of Mug20 creates the most active recombination hotspot

ade6 allele	No fusion (mug20+)	Mug20-LacI (mug20-231)	LacI-Mug20 (mug20-252)	Mug20-LacI’ (mug20-254)
3101	89 ± 13 (8)	529 ± 27 (8)	713 ± 107 (5)	576 ± 106 (4)

Intragenic recombinant frequency (Ade⁺ per 10⁶ viable spores) was assayed with ade6-3101 and ade6-52 in either the absence or presence of the indicated LacI fusions with Mug20. ade6-3101 contains 8 copies of the lacO operator substituted for part of the ade6 gene (Figure 1).

Data are mean ± SEM from (n) crosses. See Table S6 for data with additional ade6::lacO alleles.