Table 5.
ade6-3101 hotspot recombination requires Rec proteins required for wild-type recombination
| LacI-Mug20 | Mug20-LacI | Mug20a | ||||
|---|---|---|---|---|---|---|
| rec gene deletion | ade6-3101 x ade6-52 (intragenic) | ade6 – arg1 (intergenic) | ade6-3101 x ade6-52 (intragenic) | ade6 – arg1 (intergenic) | ade6-M26 x ade6-52 (intragenic) | ade6 – arg1 (intergenic) |
| + | 518 ± 54 (5) | 15 ± 4 (3) | 718 ± 39 (8) | 49 | 3800 ± 700 | 73 |
| rec8Δ | 115 ± 16 (5) | 1.3 (2) | 103 ± 18 (4) | <5.9 | 5 ± 0.3 | 0.8 |
| rec11Δ | 201 ± 10 (5) | 3.6 (2) | NDb | ND | 7 ± 1.7 | 0.7 |
| rec10Δ | 2.5 ± 1.7 (4) | <2 (2) | 5.0 ± 2.0 (4) | <2.4 | <8 | <0.4 |
| rec12Δ | 2.4 ± 0.9 (5) | <3 (2) | 3.9 ± 1.7 (4) | <2 | <5 | 0.2 |
| rec25Δ | 233 ± 49 (5) | <3 (2) | ND | ND | 34 ± 1.6 | 3.3 ± 0.4 |
| rec27Δ | 112 ± 6 (5) | 1.4 (2) | 11.9 ± 1.8 (4) | 1 | 39 ± 2 | 2.9 ± 0.6 |
| mde2Δ | ND | ND | 5.8 ± 1.2 (4) | <2 | 31c | 0.4d |
Intragenic (Ade+ per 106 spores) and intergenic (cM) recombinant frequencies were assayed with the LacI-Mug20 fusion (mug20-252), the Mug20-LacI fusion (mug20-231), or wild-type Mug20 in the presence and absence of the indicated rec genes involved in meiotic recombination. Data are mean ± SEM from (n) crosses. For crosses with either zero or one recombinant, recombinant frequency was calculated at the upper 95% confidence interval based on the Poisson distribution.
aData for rec25Δ and rec27Δ are from (49), and mde2 data are from (58); other data are from (41) except for ade6 – arg1 in rec12Δ from (82).
bND, not determined.
cData are for ade6-M26 x ade6-469. mde2+ gave 8850 Ade+/106 viable spores.
dData are for leu2-120 x lys7-2. mde2+ gave 14.6 cM.