Fig. 2.
Key aspects of DV patterning in L. goshimai. (a) Normal development of L. goshimai, lateral views with animal to the top. Key characteristics related to DV patterning are highlighted. Panels (b) and (b′) show the vegetal poles of early embryos, in which the characteristic four-cell arrangement patterns are labeled, that is, a more centered macromere (3D) surrounded by three small blastomeres, 2d22, 3c2, and 3d2 (in b′, 3D has divided to produce 4D and 4d). Panels (c) and (d) show the embryo with a well-established DV axis: shell field (sf) on the dorsal side and ventral plate (vp) and blastopore (the asterisk) on the ventral side, which could be marked by expression of the marker genes gata2/3 (c′), soxb (d′), and foxa (d″), respectively. Panel (e) shows a veliger larva in which the organs induced by the organizer (shell, foot, and eye) are indicated. pt, prototroch. The bars represent 50 μm.