Figure 2.
Designed sequences and hybridization results for LR-chimeras with various sequences. (A) Sequences of six LR-chimeras of APP (cc-740), non-APP (cc-741, cc-742 and cc-743), and two mismatched sequences (cc-741–20 and cc-741–35, containing 20- and 35-bp mismatches (orange), respectively). The red parts show restriction enzyme recognition sites, and the underlines show continuous APP sequences. Arrows indicate the terminals before circularization. (B–F) PAGE analysis of hybridization for cc-740, cc-741, cc-741–20, cc-741–35, cc-742 and cc-743 respectively. Name of samples are shown on top of the gel. The abbreviations are shown as follows. ls: linear ssDNA strand; cs: circular ssDNA strand; lc: hybridization of liner α strand and circular β strand; cl: hybridization of circular α strand and linear β strand; cc: hybridization of two circular strands. For (D) and (E), 10 mM MgCl2 was present in the gel, and 1.0 mM MgCl2 was added in the running buffer. All the gels were kept at 10°C during electrophoresis.