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. 2021 Dec 30;50(2):684–696. doi: 10.1093/nar/gkab1283

Figure 3.

Figure 3.

Thermal stability (Tm) of circular ssDNA hybrids measured by HRM. (A–E) Melting curves and differential curves of cc-740, cc-741, cc-741–20, cc-741–35 and cc-742, respectively. [dsDNA] = 1.0 μM, [HEPES] = 10 mM, [MgCl2] = 10 mM, pH7.5. The small peaks of differential curves at higher temperatures are assigned to be the Tms of lc- or cl- duplexes formed by a linear ssDNA and a circular ssDNA. (F) Tms of cc and lc measured by HRM in 1.0 mM MgCl2 or 140 mM KCl. [dsDNA] = 0.25 μM, [HEPES] = 10 mM, pH7.5.