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. 2021 Dec 30;50(2):684–696. doi: 10.1093/nar/gkab1283

Figure 4.

Figure 4.

Gel shift assay for binding of Z22 (Z-DNA-specific antibody) and ZBP1 to LR-chimera of various sequences. (A) Z22 to cc-740; (B) Z22 to cc-741; (C) Z22 to cc-741–20 and cc-741–35; (D) ZBP1 to cc-740; (E) ZBP1 to cc-741; (F) ZBP1 to cc-741–20; (G) Z22 or ZBP1 to cc-742. 10 mM MgCl2 was present in the gel for cc-741–20, cc-741–35 and cc-742. The gel was kept at 10°C during running the gel. 0.25 μM of LR-chimera was used. For (A, B) 0.25, 0.5, 0.75, 1.0 and 1.25 μM of Z22 were used. For (D, E) 0.25, 0.625, 1.25, 1.875 and 2.5 μM of ZBP1 were used; For (C, F and G) 2.5 μM ZBP1 or 1.25 μM of Z22 was used.