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. 2021 Dec 23;50(2):885–898. doi: 10.1093/nar/gkab1223

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Developmental regulation of PCNA^mUb in Drosophila. (A) Simplified scheme of early D. melanogaster embryogenesis. Embryos were collected at different hours after eggs laying (AEL), pre-MBT (0–2 h), MBT (2–4 h) and post-MBT (3–5 and 5–7 h). (B, C) Left panels: western blot of total Drosophila embryo extracts analyzed at the indicated times with the indicated antibodies; right panels: quantification of western blot panels. Data are presented as means ± SD, n = 2. Means were compared using unpaired Student’s t-test.