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. 2021 Dec 23;50(2):885–898. doi: 10.1093/nar/gkab1223

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Absence of maternal dPolη decreases embryo’s hatching rate. (A) Drawing of the experimental plan employed to evaluate the importance of dPolη during Drosophila early embryogenesis. In this experimental setup, dPolη is provided only after MBT upon zygotic gene activation. (B) Left panel: quantification of embryos displaying mitotic defects (n = 2); right panel: representative images of mitotic defects observed in either wild-type (+/+) or maternally depleted (dpolη^Exc2.15/dpolη^Exc2.15) flies. Data are presented as means ± SD. Means were compared using analysis of one-way ANOVA. (C) Statistical analysis of embryo-to-larva hatching rate of Drosophila embryos laid by mothers bearing the indicated dpolη genotypes. Embryos were collected over 12 h and incubated at 25°C for 2 days before calculating hatching rate. Means and standard deviation of unhatched embryos are expressed as error bars. Means were compared using analysis of one-way ANOVA followed by unpaired Student’s t-test for the first panel (from left to right) and Fisher’s exact test for the second panel (n = 3). (D) Statistical analysis of larva-to-adult transition hatching rate in the absence (maternally depleted) or presence (maternally provided) of maternal dPolη (left panel), and comparison between two different dpolη mutants (right panel). Data are presented as means ± SD. Means were compared with unpaired Student’s t-test (n = 3).