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. 2022 Jan 8;50(2):915–936. doi: 10.1093/nar/gkab1257

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Importance of SSEs and Rad proteins in CUP1 CNV. (A) Southern analysis of CUP1 copy number in 3xCUP1 wild-type (wt), mus81Δ, yen1Δ and mus81Δ yen1Δ cells after 10 generations ±0.3 mM CuSO₄. Quantification shows the percentage of alleles deviating from the parental copy number, P-values calculated by one-way ANOVA. Copper adaptation was assessed by treated cells with varying concentrations of CuSO₄ and incubating for 3 days. Final OD₆₀₀ was plotted against [CuSO₄] and copper tolerance quantified as area-under-curve for each culture; P-values were calculated by one-way ANOVA; n = 4. (B) Southern blot analysis of CUP1 copy number and adaptation test in 3xCUP1 wild-type (wt), mus81Δ yen1Δ, pol32Δ and mus81Δ yen1Δ pol32Δ cells after 10 generations ±0.3 mM CuSO₄, analysed as in (A), n = 6. (C) Southern blot analysis of CUP1 copy number and adaptation test in 3xCUP1 wild-type (wt), rad51Δ, rad52Δ and rad59Δ cells after 10 generations ±0.3 mM CuSO₄, analysed as in (A), n = 12.