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. 2022 Jan 25;17(1):e0262854. doi: 10.1371/journal.pone.0262854

Fig 1. Immunohistochemical staining of chondroitin sulfate in rat placenta and umbilical cord.

Fig 1

(A-I) Representative photomicrographs of the staining of anti-CS-56 antibody in rat placenta (A-C) and umbilical cord (D-F). Boxes A, D, and G are presented as the photographs B, E, and H/I, respectively. Fig 1C is a higher magnification image of Fig 1B. Immunoreactivity of CS was observed, especially in intima and media of vasculature (A-F, arrows in C and E), which disappeared by CS removal on treatment with Chase ABC (G-I, arrow in H and I). (J-O) Representative photomicrographs of the staining of anti-CS-A (2H6) antibody in rat placenta (J, K) and umbilical cord (L, M). Boxes in J, L, and N are presented as photographs of K, M, and O, respectively. Similarly, immunoreactivity was seen in intima and media of vasculature (J-M, arrow in K), and it disappeared by CS removal with Chase ABC (N, O, arrow in O). Bar, 500 μm (A, D, G, J, N), 100 μm (B, E, H, L), 50 μm (C, F, I, K, M, O).